Antibody Conjugation Kits

Chromalink Biotin Labeling Reagent, DMF soluble (MVS-B-1001-010)
The new ChromaLink™ Biotin 345S is faster and easier than the traditional and destructive HABA:Avidin displacement biotin measurement method. Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink™ Biotin 345S accurately determines the degree of biotin incorporation without any of these disadvantages. This protein-labeling reagent, also available in an easy-to-use kit (MVS-B-9007-105), contains three important features that make it ideal for biotinylation: A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems Applications include: Biotinylate protein or peptides Quantitate the extent of biotin labeling Optimize biotin-avidin complexes Highlights: Fast and easy method to biotinylate and determine the amount of labeling Label and determine the extent of biotin labeling with one reagent Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide) Nondestructive assay does not destroy biotinylated sample Kit version contains sufficient material to biotinylate 5 – 50 mg of protein and delivers 85% labeled protein recovery with essentially no contamination from non-reacted or hydrolyzed reagent.

Chromalink Biotin Labeling Reagent, DMF soluble (MVS-B-1001-010)

The new ChromaLink™ Biotin 345S is faster and easier than the traditional and destructive HABA:Avidin displacement biotin measurement method. Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink™ Biotin 345S accurately determines the degree of biotin incorporation without any of these disadvantages.
This protein-labeling reagent, also available in an easy-to-use kit (MVS-B-9007-105), contains three important features that make it ideal for biotinylation:

A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling
A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications
An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems

Applications include:

Biotinylate protein or peptides
Quantitate the extent of biotin labeling
Optimize biotin-avidin complexes

Highlights:

Fast and easy method to biotinylate and determine the amount of labeling
Label and determine the extent of biotin labeling with one reagent
Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
Nondestructive assay does not destroy biotinylated sample

Kit version contains sufficient material to biotinylate 5 – 50 mg of protein and delivers 85% labeled protein recovery with essentially no contamination from non-reacted or hydrolyzed reagent.